ELECTROPHORETIC DIFFERENTIATION OF SOME TOBAMOVIRUSES
Zdravka Sholeva1, Angela Yordanova1* and Elisaveta Stoimenova2
1National Bank for Industrial Microorganisms and Cell Cultures,
1113 Sofia, P.O.Box 239, Bulgaria,
2Institute of Genetics, Bulgarian Academy of Sciences, 1113
Sofia, Bulgaria
Summary
Purified preparations
of 16 tobamoviruses including 3 strains of tobacco mosaic virus (TMV) and
13 strains of tomato mosaic virus (ToMV) were investigated by the means
of horizontal polyacrylamide gel electrophoresis (PAGE). Among the representatives
of ToMV, the strains from group “2” after Pelham demonstrated the highest
particle mobility and the ones from group “1”, causing yellow mosaic, migrated
most slowly in electric field. Besides that all the ToMV strains differed
among each other, all they moved significantly quicker than the TMV strains.
The horizontal PAGE was applied for virus detection in plant saps and for
identification of unknown isolates. The electrophoretic analysis of viral
particles had a good reproducibility and reliability of the results and
proved a possibility for fast screening of a large number of isolates.
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